A proposed baroclinic wave test case for deep‐ and shallow‐atmosphere dynamical cores

Idealised studies of key dynamical features of the atmosphere provide insight into the behaviour of atmospheric models. A very important, well understood, aspect of midlatitude dynamics is baroclinic instability. This can be idealised by perturbing a vertically sheared basic state in geostrophic and hydrostatic balance. An unstable wave mode then results with exponential growth (due to linear dynamics) in time until, eventually, nonlinear effects dominate and the wave breaks. A new, unified, idealised baroclinic instability test case is proposed. This improves on previous ones in three ways. First, it is suitable for both deep‐ and shallow‐atmosphere models. Second, the constant surface pressure and zero surface geopotential of the basic state makes it particularly well‐suited for models employing a pressure‐ or height‐based vertical coordinate. Third, the wave triggering mechanism selectively perturbs the rotational component of the flow; this, together with a vertical tapering, significantly improves dynamic balance.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/108108/1/qj2241.pd

The EMPRISES pan-European Framework:

There is a need for further integration of information systems globally for tackling Serious Organised Economic Crime (SOEC). Taking Europe as the illustration, and levering existing pan-EU (European Union) systems such as Europol's SIENA and the FIU.NET as well as national systems, further steps can be taken to provide a more coherent and coordinated approach for detecting and deterring SOEC. This aim is achievable through the EMPRISES framework, which adds value to national, SIENA and FIU.NET systems by increasing the effectiveness of communication across Europe. EMPRISES would introduce an agreed common language (taxonomy) of SOEC, including multi-lingual support. Moreover, by enriching the taxonomy with current business tools and analysis techniques through the SOEC Architecture that EMPRISES embodies, the illegitimate businesses of SOEC can be monitored and combatted

Validation of mesoscale models

The topics discussed include the following: verification of cloud prediction from the PSU/NCAR mesoscale model; results form MAPS/NGM verification comparisons and MAPS observation sensitivity tests to ACARS and profiler data; systematic errors and mesoscale verification for a mesoscale model; and the COMPARE Project and the CME

Techniques and resources for storm-scale numerical weather prediction

The topics discussed include the following: multiscale application of the 5th-generation PSU/NCAR mesoscale model, the coupling of nonhydrostatic atmospheric and hydrostatic ocean models for air-sea interaction studies; a numerical simulation of cloud formation over complex topography; adaptive grid simulations of convection; an unstructured grid, nonhydrostatic meso/cloud scale model; efficient mesoscale modeling for multiple scales using variable resolution; initialization of cloud-scale models with Doppler radar data; and making effective use of future computing architectures, networks, and visualization software

Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes

Automated planar patch clamp systems are widely used in drug evaluation studies because of their ability to provide accurate, reliable, and reproducible data in a high-throughput manner. Typically, CHO and HEK tumorigenic cell lines overexpressing single ion channels are used since they can be harvested as high-density, homogenous, single-cell suspensions. While human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) are physiologically more relevant, these cells are fragile, have complex culture requirements, are inherently heterogeneous, and are expensive to produce, which has restricted their use on automated patch clamp (APC) devices. Here, we used high efficiency differentiation protocols to produce cardiomyocytes from six different hPSC lines for analysis on the Patchliner (Nanion Technologies GmbH) APC platform. We developed a two-step cell preparation protocol that yielded cell catch rates and whole-cell breakthroughs of ∼80%, with ∼40% of these cells allowing electrical activity to be recorded. The protocol permitted formation of long-lasting (>15 min), high quality seals (>2 GΩ) in both voltage- and current-clamp modes. This enabled density of sodium, calcium, and potassium currents to be evaluated, along with dose–response curves to their respective channel inhibitors, tetrodotoxin, nifedipine, and E-4031. Thus, we show the feasibility of using the Patchliner platform for automated evaluation of the electrophysiology and pharmacology of hPSC-CMs, which will enable considerable increase in throughput for reliable and efficient drug evaluation

Rationale and study design of the MINERVA study: Multicentre Investigation of Novel Electrocardiogram Risk markers in Ventricular Arrhythmia prediction-UK multicentre collaboration

Introduction The purpose of this study is to assess the ability of two new ECG markers (Regional Repolarisation Instability Index (R2I2) and Peak Electrical Restitution Slope) to predict sudden cardiac death (SCD) or ventricular arrhythmia (VA) events in patients with ischaemic cardiomyopathy undergoing implantation of an implantable cardioverter defibrillator for primary prevention indication. Methods and analysis Multicentre Investigation of Novel Electrocardiogram Risk markers in Ventricular Arrhythmia prediction is a prospective, open label, single blinded, multicentre observational study to establish the efficacy of two ECG biomarkers in predicting VA risk. 440 participants with ischaemic cardiomyopathy undergoing routine first time implantable cardioverter-defibrillator (ICD) implantation for primary prevention indication are currently being recruited. An electrophysiological (EP) study is performed using a non-invasive programmed electrical stimulation protocol via the implanted device. All participants will undergo the EP study hence no randomisation is required. Participants will be followed up over a minimum of 18 months and up to 3 years. The first patient was recruited in August 2016 and the study will be completed at the final participant follow-up visit. The primary endpoint is ventricular fibrillation or sustained ventricular tachycardia >200 beats/min as recorded by the ICD. The secondary endpoint is SCD. Analysis of the ECG data obtained during the EP study will be performed by the core lab where blinding of patient health status and endpoints will be maintained. Ethics and dissemination Ethical approval has been granted by Research Ethics Committees Northern Ireland (reference no. 16/NI/0069). The results will inform the design of a definitive Randomised Controlled Trial (RCT). Dissemination will include peer reviewed journal articles reporting the qualitative and quantitative results, as well as presentations at conferences and lay summaries